Josef Fares is definitely a wonderful flower in the game producers’ circle. This guy can not only make a tearful and warm work like Brothers: The Legend of Gemini, but also extend the most cordial greetings to Oscar in beautiful language at the TGA award ceremony. A film director who turned to play games seems to have an unusual obsession with two-person cooperative games. From the aforementioned Brothers: The Legend of Gemini to the critically acclaimed escape game, and today’s Double Walk, Josef Fares can always pull out many novel things from these ruined themes, whether it is family, friendship or love, so that those paired players can feel it in their carefully arranged playground.

Everything will not be as good as it is. Josef Fares, who has been deeply involved in the soil of two-person cooperation, has made rapid progress in recent years. The promise of "1000 dollars for not fun" before its release has made me have great expectations for the quality of the finished product of "Two-person Travel". After the actual experience, it is no exaggeration to say that "Two in a Row" is the best work of its kind so far, both in gameplay and in the design of "two in cooperation".

The story of "Two in a Row" revolves around a couple who are deeply involved in a marriage crisis. After their relationship broke down, the disappointed daughter turned into a humanoid doll created by her with the help of a mysterious magic book. In the game, two players need to control the husband and wife separately, and embark on a fantastic journey to lift the curse and restore the body, and at the same time, under the guidance of the magic book, repair the already fragmented feelings between the two.

Since it is "Two-in-a-Row", Qi Xin’s cooperation of two people is indispensable on the way of "Walking", and the concept of two-in-one cooperation has almost penetrated into every link of the game. At the beginning of each level, players will get a key prop closely related to the theme of the map because of the different roles they choose. For example, in a level with the tree heart as the background, players who play the leading role will get a special tool that can spray tree sap and attach it to wooden materials, and a weapon that can fire matches and detonate tree sap, and almost all the fighting and decryption games in this level are carried out around one side spraying tree sap and the other side detonating. In addition, various interactive items scattered on the map also complement the props in the hands of players, which together play an important role in connecting story lines and enriching gameplay, making the game based on platform jumping as the main gameplay, resulting in a variety of unexpected gameplay such as action shooting and even air and sea battles, providing more surprises for players.

Most games with special props as the dominant position have a common feature, that is, the difficulty of the game is directly proportional to the player’s proficiency in props. However, "Two in a Row" obviously wants you to experience the whimsy of the creator, rather than honing your skills in constant failure. Except for the almost zero death penalty, all the props in the game have very easy-to-understand functions, and will not accompany the players for too long. Maybe you are still holding a sap sprayer at the last level and performing a Hollywood-style explosion with your friends. At the next level, you put on gravity shoes and become a space dancer, roaming around the stars. More importantly, the props in "A Journey for Two" are not a cursory taste, but accurately find the opportunity of each item’s appearance, and balance its proportion in the whole game, so that all the props in the game can make the best use of it and leave a very deep impression on people.

In addition to props, the design of linear checkpoints with box-style maps is also a highlight of this work. In each large-scale plot level, "Walking for Two" sets up a "theme park" close to the story scene for players-a medieval castle built by Lego toys, a snowman town in a glass ball, and a toy city with a board game style. Every detail in these scenes with great visual impact has traces of the creator’s painstaking efforts. Although "A Journey for Two" doesn’t fill every theme park with a huge amount of playable content like "Super Mario: Odyssey", it also relieves the players’ desire to explore under the long-term linear process to some extent.

In the same vein as Escape from Life, apart from the main line checkpoints and hidden elements placed on the map that need to be explored by players, Two-in-a-Row also provides players with a large number of two-person confrontation games, which cover more than a dozen types, such as voice swimming, shooting, whack-a-mole, tug-of-war, etc., and often appear on your way to the next place, which together with the main gameplay of the whole game constitutes a perfect two-person game.

Back to the plot level, thanks to the excellent role-building and Josef Fares’ excellent skills as a film director, the plot of "A Journey for Two" is definitely a good story, although it is not complicated and does not have much significance to explore in depth. In the process of manipulating the couple to cross one difficulty after another, while enjoying the joy brought by the game, the players are slowly learning and understanding the difficulties of the two as husband and wife, father and mother in life, reviewing all kinds of past, accepting each other again and repairing the broken emotional fragments as before.

Just like the players who go hand in hand in the game, the delicate family warmth and the comedy bridge that laughs and scolds complement each other, and the two emotions together form a perfect closed loop of the whole story of "Two People in a Row".

In addition, the cartoon style also makes this work make great progress compared with the realistic "Escape from Birth" in the performance of characters and the fluency of movements. In terms of hard power, with the blessing of Unreal 4 engine, whether it is environmental modeling or light and shadow special effects, the fantasy world shown in "Two in a Row" can be described as "beautiful". The use of film shooting techniques such as long shots and slow shots also makes the performance of this play quite film-like, which is worthy of players’ repeated aftertaste and feelings contained in each shot.

There is no doubt that "Two in a Row" is definitely one of the best two-person cooperative games in recent years. From the moment I plunged into this wonderful world, I was bombarded by senses from all sides all the time and enjoyed this delicious dish carefully cooked by creativity and imagination. The only doubt is, for Josef Fares and Hazelight, is "Walking for Two" the pinnacle or just the beginning?

For more information, please pay attention to:

Seal and Seal 06 are both medium-sized cars of BYD brand, and they are different in price, configuration and market performance. The following are their main differences:

1. Price: The guide price of Seal is 149,800-249,800 yuan, while the guide price of Seal 06 is 99,800-139,800 yuan. The former is positioned as a mid-to high-end model, while the latter is positioned as a low-end model.

2. Interior: Seals’ seats are made of leather and equipped with panoramic skylights. The first row of seats has heating and ventilation functions. The seat material of Seal 06 is leather, and it is also equipped with panoramic sunroof, but the function of the first row of seats has not been announced. Therefore, the seal’s interior is of higher quality and better comfort.

3. Market performance: The national sales volume of seals in June was 8,138, ranking 65th, with an average daily attention of 55,979. The national sales volume of seals in June was 7625, ranking 69th, with an average daily attention of 122083. It can be seen that the market performance of Seal 06 is better, which may attract more consumers because of its lower price.

4. Space: The body size of the seal is 4980*1890*1495, the wheelbase is 2900, and the number of seats is 5. Seal 06 has a body size of 4830*1875*1495, a wheelbase of 2790 and five seats. Seal is slightly larger than Seal 06 in body size and wheelbase, thus providing more interior space.

5. Power: Seals and Seals 06 both use electronic continuously variable (E-CVT) gearboxes with a maximum speed of 180 km/h.. Seal is equipped with a 1.5T 139 L4 engine, while Seal 06 is equipped with a 1.5L 101 L4 engine. The former is more powerful, but the fuel consumption may be relatively high.

In a word, Seal and Seal 06 are different in price, interior, market performance, space and power. Seals are positioned in the middle and high end, with more luxurious interiors, more space and stronger power; Seal 06, on the other hand, is positioned in the middle and low end, and its market performance is good, but it is slightly inferior to seal in interior and power. Consumers can choose the right model according to their own needs and budget.

　　Wenzhou Net News The ongoing two national conferences have brought public data security into the public eye. Hu Chengzhong, deputy to the National People’s Congress and chairman of the board of directors of Delixi Group, suggested that with the epidemic prevention and control entering a new stage of "Class B and B management", the "health code" has been completely stopped, and the relevant data collected by the "health code" should be deleted within a limited time to reassure the people.

　　In Wenzhou, everyone is most familiar with the "Wenzhou Epidemic Prevention Code". It is equivalent to the merger of "health code" and travel card, and was officially launched on November 1, 2021. Relevant reports show that the number of users of Wenzhou Epidemic Prevention Code reached 8.96 million only two months after it was launched, and by April 2022, the number of users reached 14.39 million, and the number of bright codes per day reached more than 18 million.

　　In the prevention and control of epidemic situation, "health code" played a great role and became a highlight of China’s anti-epidemic experience. At present, where should the massive data behind the "health code" go?

　　Completely delete the square

　　Wang Xianming, deputy dean and professor of the School of Data Science and Artificial Intelligence of Wenzhou Institute of Technology, clearly understands his point of view: absolutely support, and the "health code" data should be completely deleted.

　　He believes that on the one hand, from the perspective of the necessity of data retention, epidemic prevention and control has entered the "Class B B Management", and there is no reason to use these data again, so there is no need to keep them. On the other hand, data storage is bound to have security risks, although it depends on where the data exists. Is the platform reliable? But this only shows that the security risk of data leakage is big or small, and it can’t completely avoid the risk.

　　"The importance of personal data is self-evident, so when deleting it, we should pay more attention to the security of the data during the destruction process, and ensure that the data is completely destroyed." Wang Xianming said.

　　Also supporting the positive view is a professional who has been fighting in the front line of epidemic prevention for three years. He believes that for the traceability work, collecting personal data in epidemic prevention and control is to quickly trace the trajectory of infected people in the past 7 days and 14 days in the event of an epidemic. This supports the epidemiological investigation to the maximum extent. Through the data, we can basically judge where the infected people are infected, and provide reference for the subsequent delineation of medium and high-risk areas. However, the professional also pointed out that after the epidemic entered a new stage, it is no longer necessary to trace the source as before, so it is no longer necessary to rely on these historical data at present. "After all, this is the transfer of personal privacy made by the public in order to cooperate with the overall situation of epidemic prevention."

　　What do ordinary citizens think? Let’s see what the supporters in the positive camp say — —

　　Citizen Li Tao: No suggestion, delete it immediately. The mission of "health code" has been completed, and relevant data should be completely deleted.

　　Citizen Qiao Qiao: I am a 2018 undergraduate student. I was often locked in school from my sophomore year until graduation. During the period of school closure, every day, in addition to doing nucleic acid, the "health code" was checked. Now when I see the "health code", I will think of that painful memory, especially hoping to completely delete the "health code" and related data.

　　Natural extinction of the opposing side

　　A data engineer, who asked not to be named, believes that the key issue is not whether the data should be saved, but what can be done to make the data more secure.

　　In this regard, Xu Gang, Dean of the School of Artificial Intelligence of Zhejiang Security Vocational and Technical College, also said that it is not easy to generate massive data on specific topics, and it is of high value in professional fields. Massive data should be preserved for a long time, and it will be better through natural extinction. He explained that theoretically, every kind of data has a life cycle, which is divided into collection — Handling — Transmission — Exchange — Six stages of destruction. In the short term, it is necessary to consider that the destruction needs to be based on certain policies, regulations and technical means, and not only will there be risks that cannot be traced back, but also corresponding costs and expenses will be incurred. However, natural extinction, such as expired batch destruction, storage equipment scrapping, natural disaster destruction and other factors, is conducive to the long-term reuse of data, especially the epidemic data can be used for long-term research on citizens’ health, epidemic spread theory, optimization methods of epidemic prevention measures, non-compliance behavior during the epidemic, etc. Of course, it is necessary to strictly ensure data security in this process.

　　There are also many citizens’ voices in the anti-party camp — —

　　Netizen "Wendy": As long as it is not leaked, the data can be kept reasonably, and it doesn’t matter if it is not deleted. In case of special circumstances in the future, these data can be used as historical situations for analysis, which may play an emergency role.

　　Citizen Chen Xi: I remember that many people were sending friends to commemorate the trip card when it was off the line. Although there were countless "health code" checks and negative nucleic acid certificates during the epidemic, there were still many warm moments during the epidemic. I hope to keep the "health code" and the data inside, which can be regarded as a commemoration of this special period.

　　What do you think of whether the data of "health code" should be deleted?

　　Source: Wenzhou Daily

　　Original title:

　　The proposal of Wenzhou delegates to the NPC and CPPCC triggered a debate?

　　Do you want to delete the "health code" data?

　　Pro: completely delete VS anti: natural extinction

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Attachment 1 Requirements for Application Materials for Senior Professional Title of Traffic Engineering (1).doc

Attachment 2 Requirements for Application Materials for Evaluation of Senior Professional Titles in Traffic Engineering (2).doc

Attachment 3 Payment Requirements for Senior Professional Titles of Traffic Engineering. wps

　　It has been half a month since the normalization of nucleic acid screening in various places. As the weather turns hot, the work of nucleic acid sampling and sample transportation becomes more difficult. Experts said that although the working environment is even worse, especially for some outdoor sampling points, the sample collection, storage and transshipment work should still be carried out in accordance with the specifications and should not be slack.

　　Battery car transfer sample

　　June 15th is the first hot day since Shanghai entered Mei, with the temperature climbing to 36 degrees and the outdoor ground temperature even higher. The first financial reporter saw in an open-air nucleic acid detection point in Shanghai that the temporary tent could not stop the scorching sun, and the sampling personnel wore protective clothing, and their faces were sweating, while there were no electric fans or cooled ice cubes outdoors. Also exposed to the scorching sun are tubes of nucleic acid reagent samples being collected.

　　The sampling time at this sampling point is from 1: 00 to 4: 00 in the afternoon. After the sampling stopped at 4: 00, the staff put the last batch of samples into a suitcase-sized small refrigerator, which was close to 4: 30. A courier arrived at the testing point by car battery car, ready to take them away.

　　I saw that the picker opened the refrigerator and hastily stuffed nearly 20 tubes of nucleic acid reagents in sealed bags into black shoulder bags, while the battery car was filled with yellow medical waste bags collected from multiple inspection points.

　　Now he will carry the samples and medical wastes to a nearby "transfer station", and then these uniformly collected samples will be transferred to the designated testing laboratory by special vehicles. He claimed that this was the sixth time he had transshipped samples today, three times in the morning and three times in the afternoon, thus ending the six-hour working day agreed in the contract.

　　According to the transshipman, the point where he collects samples every day is not completely fixed, and sometimes an extra sampling point is added temporarily, which leads to the delay of his arrival at the next point, so the actual working time is more than 6 hours.

　　A sampler of normalized nucleic acid kiosks told the First Financial Reporter that in principle, a batch of samples will be transported away every hour, but in practice, the time is not so fixed, mainly depending on whether there are enough people to transport them. Their main tool is a battery car, which transports the reagents collected from several nearby testing points to the assembly point and then sends them to the testing laboratory by special car.

　　However, for the above process, an infectious disease expert with rich field sampling experience said that this sample processing method is worrying, and the sample storage and transshipment are very inconsistent with the basic norms.

　　"We who do infectious diseases know that the most important thing is that all operations must be carried out in strict accordance with norms and standards. The storage temperature and transfer time of nucleic acid samples have strict standardized processes, which is definitely not convenient." The above experts told the First Financial Reporter.

　　The guide has clearly stipulated.

　　The Implementation Guide for COVID-19 Nucleic Acid Testing Organization (Second Edition) clearly stipulates the preservation and transportation of samples, which should be kept at low temperature (2-8 degrees Celsius). In principle, samples should be collected every 2 hours for transportation, and the samples should be delivered to the laboratory 4 hours after collection.

　　On the basis of the above guidelines, Shanghai has further put forward "126" requirements for nucleic acid testing institutions, that is, collecting and transporting a batch of samples every hour, transporting the samples to the testing institutions in 2 hours by vehicles, and completing the testing in the laboratory within 6 hours. In terms of sample transfer, the time requirement is reduced by half compared with the guideline, but in fact, it is very difficult to achieve such a goal. If the sample is not transferred in time, it will not only affect the quality of the sample, but also affect the waiting time for detection.

　　In this regard, the above-mentioned infectious disease experts told the First Financial Reporter: "Since there are norms, it should be strictly implemented in accordance with the norms. For example, it is stipulated that samples should be shipped every hour or two, and someone should come to pick them up when they arrive, instead of seeing that there are enough people to pick them up. If not, just wait."

　　The expert also warned that as the weather gets hotter, if the nucleic acid reagent cannot be stored at low temperature in time during transportation, or exposed to the sun for a long time, it will increase the risk of inaccurate nucleic acid test results.

　　He told the First Financial Reporter: "You should know that ribonuclease is ubiquitous. It exists in the air and anywhere. When you open the lid of the nucleic acid test tube, it may enter the test tube. At the temperature above 37 degrees, Rnase will easily degrade RNA, and the whole process only takes 10-15 minutes. If there is a very low virus load in the test tube, it will be diluted after ten mixes.

　　According to the observation of the first financial reporter at some nucleic acid detection points, in some mixed-tube tests, the actions that do not meet the operating standards include picking a person without screwing on the cover, which also increases the risk of exposing the sample to the air.

　　The above experts added that although adding some components, such as diethyl pyrocarbonate (DEPC), to the reagent can help to inhibit Rnase, these components will increase the cost of the reagent, and standard nucleic acid reagents are generally not added. "The easiest way is to keep it at low temperature and transport it in time. Even using ice cubes to cool down is helpful. Ice cubes have no cost and can be reused." He told the first financial reporter.

　　He also stressed that the basic operation in transferring samples is to put the samples into two-layer sealed packages and avoid the test tubes from tipping, so as to prevent the reagent from leaking and polluting other samples because the cover is not tightened.

　　Professor Lu Hongzhou, president of Shenzhen Third People’s Hospital, told the First Financial Reporter: "The best way to solve this problem is to collect samples regularly."

　　Professor Shen Hanming, an expert in cell biology at the University of Macau, told CBN: "In theory, there is a protective solution in nucleic acid reagents, and some components can inhibit RNA degradation to a certain extent, but even so, in principle, reagents should not be stored at room temperature and high temperature for a long time."

　　Movie network news(Photo/Qian Jiayi/Yang Yaru) Although 18 years have passed, no one who has seen it will forget Tony Leung Chiu Wai’s sausage mouth, Joey Wong and Leslie Cheung’s flirting swords, the big boots on Zhong Zhentao’s head, the phrase "banana, you are a guava" and the funny performances of big names such as Jacky Cheung, Brigitte Lin, Maggie Cheung and Carina Lau. This popular film was shot in Liu Zhenwei in 27 days. Recently, Liu Zhenwei was at the premiere of the film, revealing the reason for the lack of shooting time, all because of Wong Kar-wai’s harm.

　　"East into West is a movie I hate most, because I had to finish it in 27 days without a script, which was really painful." Liu Zhenwei looked a little sad. Referring to the reasons for being forced to be helpless, Liu Zhenwei revealed that it was all because of the snail speed of Wong Kar-wai’s filming. "I wanted to shoot &lsquo at the earliest; Ashes of time ’ The story when I was young, and then I called Wong Kar-wai. He said that it was very good. After we found the investment, Wong Kar-wai was responsible for shooting the first episode, and I will shoot the second episode. "

　　Liu Zhenwei sighed, "As a result, a month passed, and I went to Wong Kar-wai’s crew to watch it again. He was still filming the scene on the day when he turned on the phone 30 days ago. I was so anxious that I was sweating. Later, the date of film delivery was approaching, and Wong Kar-wai said to let me shoot a top film quickly, so that he could finish the filming, so I only spent 27 days filming" East into West "and didn’t expect the film to sell well.

　　As for the new version, Liu Zhenwei said, "Although I hate it the most, a reporter told me a few years ago that she would watch this movie regardless of her emotions, which moved me very much, so I told her that I would shoot a new one for them, so there was 2011."

　　Liu Zhenwei said, "2011 is not just funny like the last one. This one adds a lot of warmth, and these warmth things come from Leslie Cheung. Leslie Cheung and I are very good friends, and I hope my brother can see 2011, which is also a memorial to him. " Liu Zhenwei said emotionally, "I hope everyone can feel the spirit of Liu Zhenwei: always love the people and things around us."

　　It is reported that "2011 East into West" will be released nationwide on November 24th, which brings together Zhong Zhentao, Karen Mok, Eason Chan, Crystal, Deng Lixin, Kimi, Sitar tan and other stars.

　　Changsha Evening News (all-media reporter Peng Fang correspondent Chloe Wang Zeng Fan) Ms. Li, 50, has the greatest pleasure of cooking a sumptuous dinner for her family after work every day. A few days ago, Ms. Li participated in the physical examination of the unit. The results of low-dose spiral CT showed that "the space-occupying lesion of the upper lobe of the right lung does not rule out malignant lesions", and she was subsequently diagnosed as peripheral lung cancer in Hunan Cancer Hospital. Ms. Li wondered how she and her family could get lung cancer because they don’t smoke, and they usually have a healthy diet and a regular schedule. Wang Wenxiang, director and chief physician of the Second Department of Thoracic Surgery, Hunan Cancer Hospital, pointed out that in recent years, more and more patients with peripheral lung cancer were found in physical examination, and most of them were female patients who did not smoke. The academic circles thought it was related to their long-term exposure to high-temperature kitchen fumes.

　　Some surveys have found that women’s long-term exposure to high-temperature fumes will increase their risk of lung cancer by 2-3 times. Among non-smoking female lung cancer patients, more than 60% have long-term exposure to kitchen fumes; 32% women like to fry food in high-temperature oil when cooking, and the kitchen doors and windows are closed or poorly ventilated.

　　Wang Wenxiang pointed out that different cooking oils have different smoke points, and the higher the smoke point, the healthier the cooking oil. Among the commonly used edible oils, camellia seed oil has a high smoke point and can be used as a healthy first choice for cooking. In the cooking process, try to use medium fire, and control the temperature when cooking, so as not to smoke as the limit, which can not only reduce the damage of fatty acids and vitamins caused by high temperature, but also minimize the generation of smoke; Try to choose a pot with a thick bottom for cooking, so as to reduce a lot of oil smoke generated by the sharp rise of temperature in the wok; Try to use cooking methods such as steaming, boiling and stewing to reduce the oil smoke caused by frying; Ensure good ventilation in the kitchen, clean the range hood regularly, and wear a mask when necessary to reduce the smoke inhalation.

　　Wang Wenxiang reminded that the clinical cure rate of early lung cancer is over 80%, especially for women’s early lung cancer, most of which are peripheral lung cancer. Thoracoscopic surgery can achieve radical cure of lung cancer through 1 ~ 3 keyhole-sized operation incisions, and the curative effect is not inferior to that of large incision open surgery.

guide reading

On September 4th, the newly-developed recombinant novel coronavirus vaccine for inhalation (adenovirus type 5 vector) was put forward by the National Health and Wellness Commission, and was approved by National Medical Products Administration to be used as booster for emergency use, making it the first inhaled COVID-19 vaccine in the world.

On October 25th, Shanghai started the registration of intensified immunization with recombinant Covid-19 vaccine for inhalation (adenovirus type 5). On 26th, the immunization with recombinant Covid-19 vaccine (adenovirus type 5) for inhalation was started.

What is recombinant Covid-19 vaccine for inhalation? This non-injected, non-cold chain COVID-19 vaccine was called "revolutionary ideal" by Academician Chen Wei. What’s good about this "ideal" vaccine?

Chen Wei, researcher of Academy of Military Sciences, vice chairman of China Association for Science and Technology and academician of China Academy of Engineering.

↓↓↓

Studies at home and abroad have proved that enhanced immunization with Covid-19 vaccine can enhance the protection effectiveness and prolong the protection period.

The so-called atomization inhalation immunity means that the vaccine is atomized into tiny particles by an atomizer, and then enters the respiratory tract and lungs by breathing inhalation, thus stimulating mucosal immunity, which cannot be brought by intramuscular injection. Generally, intramuscular injection of COVID-19 vaccine can only induce humoral immunity and cellular immunity. In addition, immunization with atomized inhalation is painless and has higher accessibility.

Immune response and specific antibodies induced by intramuscular injection are easier to enter the blood circulation system, but their contents in nasal cavity and lungs are not high enough to achieve immune effect quickly when the virus invades. It takes a certain reaction time for these antibodies to reach the respiratory tract from the blood, but the virus invades our body and usually seizes this time difference, so many people are infected during this time.

However, this non-injected vaccine is inhaled, and only one-fifth of the dose of the vaccine is injected each time. It can also induce mucosal immunity through atomized inhalation to achieve the best effect of triple immunity, which can not only block the infection of Covid-19, but also inhibit the spread of the virus.

According to the relevant requirements of the state, citizens only need one dose of hypohomologous booster immunization or sequential booster immunization at present. When people aged 70 and over are vaccinated, they should be accompanied by family members or volunteers.

COVID-19 vaccine for inhalation 18 questions

1. What is recombinant Covid-19 vaccine for inhalation?

Answer: The recombinant Covid-19 vaccine for inhalation (adenovirus vector type 5) uses an adenovirus vector harmless to human body to deliver the genetic material expressing S protein into human body, and uses an atomizer to atomize the vaccine into tiny particles, which can reach the lung tissue through oral breathing by inhalation, thus stimulating triple immunity of mucosa, body fluid and cells.

2. What are the conditions for inoculation of recombinant Covid-19 vaccine for inhalation?

A: At this stage, the inactivated vaccine produced by Sinopharm China Biotechnology Co., Ltd., Beijing Institute of Biological Products Co., Ltd., Sinopharm China Biotechnology Co., Ltd., Wuhan Institute of Biological Products Co., Ltd. and Beijing Kexing Zhongwei Biotechnology Co., Ltd. and the intramuscular recombinant COVID-19 vaccine (adenovirus type 5) produced by Kangxinuo Biological Co., Ltd. can be used to carry out one dose of booster immunization for people aged 18 and above for six months. Recombinant Covid-19 vaccine for inhalation (adenovirus type 5 vector) is not used for basic immunization for the time being.

All kinds of people only need to be vaccinated with one dose of hypohomologous booster immunization or sequential booster immunization.

3. What is the dose of recombinant Covid-19 vaccine for inhalation?　

A: The dosage is 0.1mL each time.

4. Can people over 60 be vaccinated? Can people under 18 be vaccinated with recombinant Covid-19 vaccine for inhalation?

A: At present, the recommended vaccination population for inhalation dosage forms is people aged 18 and above.

5. How effective is the booster immunization of recombinant Covid-19 vaccine for inhalation?　

A: At present, the clinical study of sequential booster immunization with recombinant Covid-19 vaccine for inhalation and inactivated novel coronavirus vaccine in healthy adults aged 18 and above in China shows that sequential booster immunization with recombinant Covid-19 vaccine for inhalation and inactivated vaccine can significantly enhance the immune response.

6. What is the immune persistence of recombinant Covid-19 vaccine for inhalation?

A: Immunization persistence research shows that after 6 months of heterologous inoculation with recombinant Covid-19 vaccine for inhalation, the neutralizing antibody remains at a high level.

7. What is the safety of recombinant Covid-19 vaccine for inhalation?

A: A safety study conducted among tens of thousands of people showed that the recombinant Covid-19 vaccine for inhalation was safe for people over 18 years old, and no serious adverse reactions were found.

8. What are the vaccination contraindications of recombinant Covid-19 vaccine for inhalation?

Answer: (1) Those who are allergic to the active ingredients, any inactive ingredients and substances used in the production process, or those who have been allergic to similar vaccines before.

(2) Those who have had severe allergic reactions to vaccines in the past (such as acute allergic reactions, angioneurotic edema, dyspnea, etc.).

(3) Patients with uncontrolled epilepsy and other progressive nervous system diseases have a history of Guillain-Barre syndrome.

(4) People who are suffering from fever, or acute diseases, or acute attacks of chronic diseases, or patients with uncontrolled serious chronic diseases;

(5) Pregnant women.

9. Can patients with chronic diseases be vaccinated with recombinant Covid-19 vaccine for inhalation?

A: According to the Technical Guidelines for Vaccination in Covid-19 (First Edition), people with chronic diseases are at high risk of severe illness and death after being infected with Covid-19. People with chronic diseases with stable health and good drug control are recommended to be vaccinated. Vaccination is suspended when chronic diseases are in acute attack. Have a history of convulsion, epilepsy, encephalopathy or mental illness or family history; Have a history of asthma, chronic obstructive pulmonary disease, pulmonary fibrosis and other basic diseases or abnormal lung function; Use with caution in patients with impaired immune function (such as malignant tumor, nephrotic syndrome and other immunodeficiency diseases).

10. Do people cured in COVID-19 need to be vaccinated with recombinant Covid-19 vaccine for inhalation?

A: According to the Technical Guidelines for Vaccination in Covid-19 (First Edition), it is rare for Covid-19 to be reinfected within 6 months after infection. In the past, COVID-19 virus infected people (patients or asymptomatic infected people) can be vaccinated with one dose after 6 months on the basis of full notification.

11. Can people with allergies be vaccinated with recombinant Covid-19 vaccine for inhalation?

A: In the Technical Guide for Vaccination in Covid-19 (First Edition), it is clear that those who have had severe allergic reactions to vaccines in the past (such as acute allergic reactions, angioneurotic edema, dyspnea, etc.) are contraindications for vaccination of Covid-19 vaccines; In addition, the severity of allergies and allergens should be considered and implemented according to the doctor’s advice.

12. How to inoculate recombinant Covid-19 vaccine for inhalation?

A: Please practice several times before inhaling, and then start the formal vaccination.

Step 1: Take a deep breath first (do not exhale into the atomizing cup);

Step 2: hold the nozzle of the atomizing cup in your mouth, and inhale deeply until there is no fog in the cup;

Step 3: Hold your breath for more than 5 seconds (at least 5 seconds), then breathe normally, and the inoculation is over.

13. Under what circumstances should we re-inhale recombinant Covid-19 vaccine for inhalation on the spot?

Answer: (1) There is obvious misty gas in the atomizing cup after inhalation;

(2) Cough and early exhalation occur during inhalation (including 5-second breath-holding period).

Suction failure needs to inhale a dose of vaccine again, and only one repeated inhalation is allowed.

14. What should I pay attention to after vaccination with recombinant Covid-19 vaccine for inhalation?

A: You should stay on the spot for at least 30 minutes after inoculation. Avoid contact with previously known allergens and common allergens within one week after inoculation, and try not to drink alcohol, eat spicy food or seafood. It is recommended to have a light diet and drink plenty of water.

15. What adverse reactions may occur after vaccination with recombinant Covid-19 vaccine for inhalation?

A: Common systemic adverse reactions include symptoms such as fatigue, headache, fever, dry mouth and swollen throat. Generally, they can recover by themselves without special treatment.

16. Will the inoculation of recombinant Covid-19 vaccine for inhalation affect the results of nucleic acid test and antigen test?

Answer: The characteristic of adenovirus vector COVID-19 vaccine is that it only contains the gene sequence of S protein and expresses antigen in human cells, and it does not contain the open reading frame 1ab(ORF1ab) and nucleocapsid protein (N protein) at the nucleic acid detection site, so the vaccination of this vaccine will not cause COVID-19 to be positive for nucleic acid detection.

17. Can I be vaccinated during pregnancy?

A: If you are pregnant after vaccination or have been vaccinated in the case of unknown pregnancy, based on the understanding of the safety of the above vaccines, it is not recommended to take special medical measures (such as termination of pregnancy) just because you have been vaccinated with Covid-19 vaccine. It is recommended to do a good job in pregnancy examination and follow-up. For women who have a pregnancy plan, it is not necessary to delay the pregnancy plan just because of Covid-19 vaccination.

18. After three injections, do I need to get another vaccine for inhalation?

A: According to the guidelines on vaccination work, those who have already completed the booster immunization will not be given further booster immunization at this stage.

Source: The Paper, Shanghai Publishing, Jinshan CDC, Science and Technology Daily. 　 　

　　Xinhua News Agency, Tokyo, August 24th(International observation) How to talk about honesty after a long period of planning — — Before and after Japan’s decision to discharge nuclear polluted water into the sea

　　Xinhua news agency reporter

　　According to the decision of the Japanese government, Fukushima nuclear polluted water began to be discharged into the sea on August 24. This sewage disposal process will last for decades.

　　Tracing back to the whole process of Japan’s decision to discharge nuclear polluted water into the sea, we can clearly see that discharging nuclear polluted water into the sea is its long-planned "established policy", which is an out-and-out violation of international law and an extremely selfish and irresponsible state act. As a result, the cost of handling the Fukushima nuclear accident will be passed on to the whole world.

　　Nuclear polluted water has been discharged into the sea for a long time

　　Since the Fukushima Daiichi nuclear power plant suffered a serious accident in March 2011, a large amount of high-concentration nuclear polluted water has been produced every day due to the cooling of the melted core with water and the flow of rainwater and groundwater. In April 2011, Tokyo Electric Power Company, the operator of the nuclear power plant, deliberately discharged nuclear polluted water into the sea, which aroused great concern and concern in society. In December of the same year, TEPCO said that it had formulated a plan to discharge "low-concentration polluted water" into the sea.

　　In March 2013, TEPCO’s key facility "Multi-nuclide Treatment System" (ALPS) was put into trial operation, but since then, there have been many problems: frequent water leakage, and radioactive substances such as strontium in the treated water still exceeded the standard in 2018. In 2021, it was found that nearly half of the filter screen of the exhaust port for adsorbing radioactive substances was damaged & HELIP; …

　　Since ALPS was put into operation, Japan has called the treated nuclear polluted water "treated water". In fact, of the more than 1.34 million cubic meters of nuclear polluted water in the storage tank of Fukushima Daiichi Nuclear Power Plant, more than 1.33 million cubic meters have been treated by ALPS, but only about 30% have reached the standard of "treated water" defined by TEPCO, and the so-called "treated process water" that fails to meet the standard accounts for about 70%. Another 9,000 cubic meters of nuclear polluted water has not been treated by ALPS.

　　And how to solve the final destination of these "treated water"?

　　As early as December 2013, the Ministry of Economy, Trade and Industry, Japan’s nuclear energy authority, set up a working group to conduct technical discussions on the discharge of "treated water". In June 2016, the working group released a report saying that after the evaluation of five methods, such as ocean discharge, underground burial (adding cement and then burying it underground), stratum injection (injecting it into the depth of stratum by pipeline), steam release (vaporizing it into water vapor and discharging it into the atmosphere) and hydrogen release (electrolyzing it into hydrogen and discharging it into the atmosphere), it is the "lowest cost" method to dilute the "treated water" and discharge it into the sea.

　　This report set the tone for the later sea discharge plan, but it was strongly opposed by Japanese organizations such as agriculture, forestry and fisheries after its publication. Even Masayoshi Yoshino, then Japan’s minister of recovery, said that he opposed the treatment of nuclear pollution into the sea.

　　However, TEPCO and the Ministry of Economy, Trade and Industry have clearly regarded the sea-discharging plan as an "established policy". In July 2017, the Ministry of Economy, Trade and Industry held the "Local Coordination Meeting on Waste Furnace and Pollution Water Countermeasures" in Fukushima City, posing as a gesture of consultation with the local authorities. However, before the meeting, the then president of TEPCO, Takashi Kawamura, claimed to the media that TEPCO had "made a judgment" on discharging the sea, which caused widespread social dissatisfaction.

　　In order to convince the public, the Japanese government set up a committee with experts in related fields. In August 2018, the Committee held hearings in Fukushima and Tokyo, nominally to listen to the opinions of the people, but in fact it was to endorse the sea exclusion plan. At the hearing, Toyoda, then chairman of the Japan Atomic Energy Regulatory Commission, said that "discharging the sea is the only feasible option" was questioned by all parties. For example, in response to the problem of pollution water storage capacity and lack of open space raised by TEPCO, some people pointed out that 100,000-ton large-scale oil storage tanks can be considered, and the Fukushima Second Nuclear Power Plant, which has identified waste furnaces, can be used for open space. In view of the technical difficulty of nuclear pollution water treatment, it is suggested that the water vapor emission method used in the 1979 Three Mile Island nuclear accident in the United States can be adopted. It is also pointed out that the separation technology of radioactive element tritium which cannot be removed by ALPS is under study and should be discharged after the technology is mature and applied.

　　However, in February, 2020, the above-mentioned committee published a report saying that there are "many problems" in formation injection, underground burial and hydrogen release, and that sea discharge and steam release with precedents are the "realistic options", while emphasizing that sea discharge has "many benefits" over steam release.

　　In April 2021, the Japanese government unilaterally announced that it would discharge nuclear polluted water into the sea in 2023, ignoring domestic and foreign objections. Since then, the preparatory work for sea discharge has begun to advance in full swing: in December 2021, TEPCO submitted a construction plan for water discharge equipment to the Atomic Energy Regulatory Commission; In July 2022, the Atomic Energy Regulatory Commission approved the plan; On January 13 this year, the Japanese government confirmed that it will implement the sea discharge in the "spring and summer"; On June 26, TEPCO announced the completion of the construction of sea discharge equipment; On July 7, the Atomic Energy Regulatory Commission delivered the "Certificate of Acceptance" of the sea discharge facilities to TEPCO.

　　Self-directed and self-performed "authoritative certification"

　　On July 4th this year, Grossi, Director General of the International Atomic Energy Agency (IAEA), visited Japan and submitted a comprehensive assessment report on the disposal of nuclear polluted water in Fukushima to Japanese Prime Minister kishida fumio. According to the report, Japan’s sea-discharging scheme is generally "in line with international safety standards", so Japan claims that the safety of the scheme has obtained "authoritative certification".

　　However, there are many questions about the fairness and scientificity of this report.

　　First of all, the Japanese side first made a decision to discharge the sea, and then entrusted IAEA to make a safety assessment. Obviously, the purpose is not to find a scientific and reasonable solution, but to use the agency to endorse the sea discharge plan.

　　According to the contents of the report, after the Japanese government announced the decision to discharge the sea in April 2021, it signed an "authorization agreement" with IAEA in July of the same year to entrust "evaluating the safety of ALPS water treatment". The evaluation object is only limited to the sea discharge scheme and does not involve other schemes. This means that the evaluation conclusion cannot prove that the sea discharge scheme is the safest and most reliable scheme.

　　Secondly, before Japan officially authorized the IAEA evaluation, it had already started the relevant layout around the "certification card".

　　In April 2013, shortly after ALPS was put into trial operation, the Japanese government invited an IAEA delegation to Fukushima. The delegation released a report a month later, suggesting that Japan start to study the emission problem. At that time, the Director-General of IAEA was the Japanese Amano. In December 2019, Grossi succeeded Amano as the Director General of IAEA, and Japan continued to work for IAEA. In March 2021, Hiroshi Daiyama, then Japan’s Minister of Economy, Trade and Industry, held talks with Grossi, requesting the IAEA to provide support for Japan in eliminating the "reputation damage" caused by nuclear pollution water discharge. On April 14th of the same year, the day after the Japanese government announced its decision to expel the sea, Hiroshi Daishan met with Grossi again, requesting IAEA’s support in environmental monitoring and explaining to the international community.

　　"Tokyo News" reported that in the past, the Japanese government paid a huge share of expenses and other payments to the IAEA, and many departments of the Japanese government sent personnel to the IAEA. These factors will inevitably have an impact on the IAEA’s assessment of the safety of Japan’s nuclear pollution water discharge plan.

　　Members of the Common Democratic Party, the largest opposition party in South Korea, pointed out in a meeting with Grossi on July 9 that it is very regrettable that IAEA did not follow the principles of neutrality and objectivity, catered to Japan’s position of discharging nuclear pollution into the sea from beginning to end, and rushed to a conclusion regardless of the impact of this practice on neighboring countries.

　　Thirdly, at the beginning of the IAEA evaluation report, it is emphasized that the opinions in the report do not necessarily reflect the views of IAEA member States, and the report is not a recommendation or endorsement of Japan’s sea discharge plan, and IAEA and its member States are not responsible for any consequences caused by the report. This disclaimer clearly shows that the report cannot represent the opinions of the international community, nor can it prove the legitimacy and legality of Japan’s plan to discharge the sea.

　　Experts from China who participated in IAEA’s technical working group on the evaluation of Fukushima’s "ALPS treated water" for sea discharge — — Researcher Liu Senlin of China Institute of Atomic Energy told the media that the IAEA secretariat had asked for the expert opinions of the technical working group on the draft evaluation report, but the time window left for the experts was very limited, and the expert opinions were for reference only, so it was up to the IAEA secretariat to decide whether to adopt them. After receiving the feedback, the IAEA secretariat hastily released the report without discussing and negotiating with experts from all sides on the revision of the report and the adoption of opinions.

　　Li Song, China’s Permanent Representative to the United Nations and other international organizations in Vienna and Permanent Representative to the International Atomic Energy Agency, pointed out that the conclusion of the IAEA report on the safety of Japan’s sea discharge program is one-sided and lacks persuasiveness and credibility. Due to the limitation of authorization, the agency failed to assess the long-term effectiveness of the Japanese purification device, to confirm the authenticity and accuracy of the data of nuclear polluted water, to ensure that the international community can grasp the situation of excessive discharge in time, and to predict the impact of long-term accumulation and enrichment of radionuclides on the marine ecological environment, food safety and public health. "Without confirmation of accurate data, reliable equipment and effective supervision, it is impossible to draw the conclusion that it is safe to discharge more than 1.3 million tons of nuclear polluted water into the ocean within 30 years."

　　Pretending to be a show, worrying about integrity

　　The Fukushima Daiichi nuclear power plant has a marine life breeding room, which keeps the common flounder along the coast of Fukushima. One of the tanks is ordinary seawater, and the other is treated nuclear polluted water, which is called "treated water".

　　From a scientific point of view, experts and environmental protection organizations have many doubts about the nuclear pollution water treatment and other related data provided by TEPCO.

　　Professor ferenc Dalnoki-Veres, an expert in nuclear physics at Middlebury Institute of International Studies in the United States, pointed out that the data provided by Japan were "incomplete, incorrect, inconsistent and one-sided". The Japanese environmental protection group "FoE Japan" pointed out various problems in TEPCO’s statement about "treating water": some radioactive elements such as iodine 129 and strontium 90 still exceeded the standard after being "treated" by ALPS; The water samples detected by TEPCO so far only account for 3% of the stored polluted water, and the test results provided by TEPCO are not representative. The "treated water" in Fukushima is in direct contact with the melted core and cannot be compared with the normal nuclear power plant drainage … …

　　What is even more worrying is that TEPCO had a "black history" of tampering with data and concealing the safety of nuclear power plants.

　　On the third day after the Fukushima nuclear accident in March, 2011, TEPCO was able to judge the core meltdown of Units 1 to 3 according to the relevant data, but it was always whitewashed with "core damage" until two months later, when it admitted the core meltdown. TEPCO later admitted that it was intentionally concealing the truth according to the instructions of the then president. TEPCO once claimed that no new nuclear polluted water was discharged into the sea after June 2011, but with the exposure of a series of leakage incidents in 2013, TEPCO finally admitted that high-concentration nuclear polluted water leaked into the sea, saying that it was not announced in time because of fear of affecting the reputation of local fisheries; In September 2021, when TEPCO reported the damage of ALPS exhaust filter, it admitted that the same filter was damaged two years ago, but it was not reported, and the reason was not investigated. It was just a matter of replacing the filter. In October 2022, TEPCO was exposed to mislead visitors with a problematic radiation detector to prove the safety of "ALPS treating water".

　　Guan Guzhi, a scholar at the University of Tokyo, also pointed out that not only in Fukushima, but also in other nuclear power plants under TEPCO, management and safety problems are constantly occurring, which makes people unable to believe its disposal capacity. "Does TEPCO have this qualification to discharge into the ocean?"

　　The dishonesty of TEPCO and the Japanese government is also manifested in the attitude of reneging. The plan of discharging nuclear polluted water into the sea has been strongly opposed by local fishermen all over Japan, especially in Fukushima. Under this circumstance, TEPCO and the Japanese government assured the Fukushima Federation of Fishery Synergies and the Japan National Federation of Fishery Synergies in August 2015 that they would not discharge nuclear polluted water into the ocean until they were understood by fishermen and other relevant parties.

　　Although the Japanese government tried every means to convince the fishermen, it never succeeded. Since 2020, the Japan National Federation of Fishery Synergies and the Fukushima Federation of Fishery Synergies have passed special resolutions for four consecutive years, resolutely opposing the discharge of nuclear polluted water into the sea. However, regardless of opposition, the Japanese government and TEPCO violated their own commitments and insisted on promoting the sea discharge plan.

　　Yasushi Sakamoto, president of the National Federation of Fishery Cooperatives, said after meeting with Yasutoshi Nishimura, Minister of Economy, Trade and Industry, on July 14th this year that it is impossible to change the opposition position as long as we are not at ease with emissions. After exchanging views with Yasutoshi Nishimura on 11 July, Nozaki Tetsuo, president of Fukushima Fisheries Cooperative Association, stressed that the fishermen could not tolerate the discharge of nuclear polluted water into the sea, in contrast to the government’s promise of "no disposal" without the understanding of relevant parties.

　　Point the deer at the horse and pretend to be false.

　　Faced with the strong opposition at home and abroad to the discharge of nuclear polluted water into the sea, the Japanese authorities launched an intensive public relations campaign to confuse the audience and publicize the "safety theory of nuclear polluted water" as a key point of Japanese diplomacy.

　　Japanese Ministry of Foreign Affairs, Ministry of Economy, Trade and Industry, Reconstruction Agency and other government departments have set up special links on the homepage of official websites to promote the safety of ALPS "water treatment". The social media accounts of the Ministry of Foreign Affairs and the Ministry of Economy, Trade and Industry also put relevant promotional videos at the top or in a prominent position, and launched multilingual versions.

　　Japan holds the rotating presidency of the Group of Seven (G7) this year. During the G7 Climate, Energy and Environment Ministers’ Meeting in April this year, Japanese Minister of Economy, Trade and Industry Yasutoshi Nishimura declared at the press conference that "including ‘ Treated water ’ The steady progress of waste furnace work, including discharging into the sea, was welcomed. As a result, German Environment Minister Lemcke said on the spot that "discharging (nuclear polluted water) into the sea is not welcome". The Japanese side originally tried to insert "Welcome to the transparent process of discharging the sea" into the joint communique of the meeting to show "international recognition". This statement was opposed by Germany, but the Japanese side still used the host status to write "Welcome … … Japan and IAEA’s transparent efforts based on scientific evidence "and" supporting IAEA’s independent review ". The same content later appeared in the joint communique of the leaders of the Hiroshima Summit of G7 in May.

　　The Japanese side also launched a public relations offensive against Pacific island countries. These island countries suffered greatly from the nuclear test of the United States in the Pacific Ocean in the past, but now there is a strong opposition to the discharge of nuclear polluted water from Fukushima, which has become the key "appeasement" object of Japan. According to the report released by the Ministry of Economy, Trade and Industry on July 31, Japan has conducted "explanation work" for all members and regions of the Pacific Island Forum since February this year.

　　The Japanese side also frequently held briefing sessions for diplomats and foreign journalists from various countries in Japan, and tried every means to publicize the "safety" of nuclear polluted water discharging into the sea. In Japanese explanations, especially in foreign language versions, "treated water" is generally used to refer to nuclear polluted water, with the intention of diluting its pollution characteristics and potential harm to confuse people. According to some foreign journalists in Japan, once there is any content in their reports that questions the safety of nuclear polluted water discharge, TEPCO and Japanese officials will call and write to put pressure on it.

　　It is worth noting that according to the IAEA’s safety regulations on the discharge of radioactive materials into the environment, authorization for the discharge of radioactive materials should provide information and consultation to the affected stakeholders. "Some interested parties may be in other countries, especially neighboring countries." However, in the face of opposition and doubts from neighboring countries, the Japanese side did not communicate sincerely, but made a mistake and slandered the legitimate concerns of regional countries about the marine environment and food safety as "playing political cards." Some right-wing media in Japan even dressed Japan as a "victim" and angrily threatened to "counter" neighboring countries that raised objections to the discharge of nuclear polluted water into the sea.

　　On July 4th, the Embassy of China in Japan expounded China’s position on the issue of Fukushima nuclear polluted water being discharged into the sea, pointing out that Japan’s so-called "hope for dialogue and consultation with China" was insincere. So far, China has carried out exchanges with Japan in bilateral and multilateral channels and repeatedly expressed the opinions and concerns of professional departments. However, regardless of China’s position, Japan insists on promoting sea discharge according to the established timetable. "If the Japanese side regards sea exclusion as the premise of consultation and blindly imposes sea exclusion on China, what is the significance of such consultation?"

　　The discharge of Fukushima nuclear polluted water into the sea is not a private matter or a trivial matter of Japan, but a business matter and a major event related to the marine environment and human health. The Japanese government ignored the legitimate concerns of the international community, violated its due international obligations, and forced nuclear pollution water into the sea, endangering the marine environment and human health and infringing on the legitimate rights and interests of neighboring countries. It is by no means a responsible country.

Cancer, as one of the most common diseases with high mobility and high mortality in the world, the clinical treatments for its tumors generally include surgery, chemotherapy and radiotherapy. Due to the shortcomings of chemical drugs and various toxic and side effects, people have been committed to developing more effective drug delivery systems for cancer treatment, with the aim of reducing side effects and ensuring its efficacy.

With the progress and maturity of nanotechnology, its advantages are gradually reflected.

Introduction of nano-drugs

Diagram of sustained release process of nano-drugs

Nano-drug and its delivery system

Nano-medicine refers to nanoparticles or nano-carriers, which is a new product produced by the combination of nano-technology and medicine.

Nano-drug delivery system refers to the integration of small drug molecules into nano-materials by means of encapsulation or adsorption.

Advantages of nano-drugs

First, improve the solubility and absorption of drugs.

Nano-sized drugs are different from most chemotherapy drugs. Nano-sized drugs have larger specific surface area, better solubility, faster dissolution, improved absorption properties and better therapeutic effect.

At the same time, due to better solubility, the dosage of patients can also be appropriately reduced.

Second, prolong the drug’s circulation time in vivo, and enrich it to the focus.

Through some modification and treatment on the surface of nano-drugs, the circulation time of drugs in human body can be prolonged, which is beneficial for drugs to reach the lesion site, and special modification may make drugs more concentrated in the tumor site, resulting in better selectivity and targeting properties.

Nano-drugs can enter the tumor tissue through the porous blood vessels of the tumor, and achieve a high degree of penetration and retention in the tumor. Therefore, nano-preparation has excellent properties of high drug loading rate, passive targeting and controlled drug release.

Clinical application of nano-preparation.

Paclitaxel albumin nanoparticles

Paclitaxel is a commonly used anticancer drug in clinic. Here is a brief introduction to the nano-preparation of paclitaxel:

Paclitaxel, a commonly used anticancer drug

Albumin nanoparticles

Albumin nanoparticles are nano-particles based on albumin.

Albumin is an endogenous natural product with the advantages of safety, non-toxicity, non-immunogenicity, biodegradability and good biocompatibility. Moreover, the nanoparticle carrier system has unique targeting, sustained and controlled release characteristics and drug protection.

By high-pressure homogenization, paclitaxel and human serum protein preparation were made into nanometer freeze-dried powder, which can improve the solubility, avoid a large number of paclitaxel nanoparticles aggregation, and help the drug to be absorbed into tumor cells and give full play to its role.

Pharmacological action of paclitaxel

Paclitaxel is an anti-microtubule drug, which can stabilize microtubules by promoting the polymerization of tubulin dimer and inhibiting its depolymerization, thus inhibiting the normal dynamic reorganization of microtubule network during interphase and mitosis.

In addition, paclitaxel can lead to abnormal arrangement of microtubule "bundles", affect the division of tumor cells and promote tumor cell apoptosis during the whole cell cycle and cell mitosis.

indication

The application of albumin nanoparticles in the in vivo delivery of paclitaxel and cancer treatment has become a hot topic in recent years.

Albumin paclitaxel nanoparticles are mainly suitable for the treatment of metastatic breast cancer that failed in combination chemotherapy or breast cancer that recurred within 6 months after adjuvant chemotherapy.

Unless there are clinical contraindications, an anthracycline anticancer drug should be included in previous chemotherapy.

matters need attention

In the process of using paclitaxel albumin nanoparticles, it should be noted that paclitaxel for injection (albumin binding type) also has adverse drug reactions and must be used under the guidance of a doctor with chemotherapy experience.

Only with perfect diagnosis and treatment facilities can the complications and adverse reactions caused by drugs in treatment be effectively controlled.

references

[1] Jie Chen, Cao Ye, Li Shuangshuang, Luo Lailong, Yu Peng, Li Mingyuan. Research progress of novel nano-drug delivery system for tumors [J]. China Journal of Pharmacy, 2020,55(21):1749-1756.

Xin li, Zhu Dandan, Chen Yu, Lin Shuting, Han Lili, Li Ying, Liu Xiaoxuan. Research progress of nano-drug delivery system in promoting deep penetration of tumor [J]. Zhongnan Pharmacy, 2020,18(12):2009-2018.

Zhao Shuang, Zhang Shaofang, Mu Xiaoyu. Research progress of nano-drugs [J]. Tianjin Pharmacy, 2020,32(02):57-61.

Chen Dongxiao, Zhang Juanmei, Li Jianye, Xu Tianhong, Dong Yaqin, Pu Xiaohui. Research status and progress of paclitaxel albumin nanoparticles [J]. Journal of Henan University (Medical Edition), 2017,36(04):296-300.

[5] Chen Kang. Analysis of the research progress of paclitaxel pharmaceutical preparations [J]. Heilongjiang Medicine, 2019,32(06):1390-1392.

Contributed by: Song Haolin (Ruijin Intern Pharmacist)

Original title: "How do nano-warriors fight cancer-do you understand? 》

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